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pcdna3 1 catalase  (Thermo Fisher)


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    Thermo Fisher pcdna3 1 catalase
    Pcdna3 1 Catalase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3%2E1/CATALASE/10__1016_slash_j__mrgentox__2026__503933-80-14-18
    Average 96 stars, based on 1 article reviews
    pcdna3 1 catalase - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Virus:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Amplification:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Polymerase Chain Reaction:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Sequencing:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Expressing:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Plasmid Preparation:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Derivative Assay:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Modification:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Purification:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Cell Culture:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Transfection:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Clone Assay:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Produced:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Luciferase:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.

    Control:

    Article Title: DNA plasmid-launched live-attanuated vaccines for plus-sense singel stranded RNA
    Article Snippet: Next, the simian virus 40 (SV40) or cytomegalovirus (CMV) promoter sequences were amplified by standard PCR from the pcDNA3.1(+) (ThermoFisher Scientific) and fused with the 5′UTR sequence of ZIKV, respectively.

    Article Title: Molecular diversity of protostome non-visual opsin arthropsin
    Article Snippet: The cDNA of crab-eating macaque ( Macaca fascicularis ) red-sensitive cone pigment ( AF158968 ) was tagged with the epitope sequence of Rho1D4 at the C-terminus and was introduced into pcDNA3.1 (Thermo Fisher Scientific).

    Article Title: Anti-human NR1 antibody derivative
    Article Snippet: A linker, a HisTag sequence (His*8), an AviTag sequence (Avidity) and a nucleotide sequence encoding a stop codon were added to the 3′ terminus of a polynucleotide encoding the extracellular domain of human NR1 (SEQ ID NO: 43, corresponding amino acid sequences is shown in SEQ ID NO: 44), by gene engineering techniques, and inserted into pcDNA3.1 (Thermo Fisher Scientific), and became an expression vector of fusion protein of human NR1 and HisTag (hereinafter referred to as “human NR1-His protein”).

    Article Title: Wireless, battery-free vagal electrical stimulation: A novel approach to inhibit cardiac hypertrophy via H3K18 lactylation mediated mitophagy.
    Article Snippet: Cells were seeded in a 24-well plate to reach 80 % confluency after 24 h. Transfection was performed using Lipofectamine 3000 (Thermo Fisher Scientific) with a plasmid mixture containing 1 μg pcDNA3.1, 1 μg pGL3 (firefly luciferase reporter), and 0.1 μg pRL-TK (renilla luciferase internal control) per well, and combined with 6 μl Lipofectamine 3000 in Opti-MEM.

    Article Title: Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.
    Article Snippet: The same method was also used to integrate attB regions upstream and downstream the complete DsuzIR75d ORF, which was amplified from the human HEK-codon-optimized sequence of DsuzIR75d cloned into pcDNA3.1 produced by GeneArtTM gene synthesis service (Thermo Fisher Scientific, Waltham, MA, USA) (from now on, DsuzIR75dHEK).

    Article Title: miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway.
    Article Snippet: This study was to investigate the mechanism of miR34c5p in alleviating kidney injury in diabetic nephropathy (DN) rats by targeting ROCK1 and MAPK/ERK signaling pathway.. The rat model of DN was established and fasting blood glucose, 24-h proteinuria, blood urea nitrogen, and serum creatinine were measured to quantify kidney injury.. Kidney tissue was dissected for H&E and TUNEL staining.

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
    Article Snippet: C-terminal Flag-tagged CHMP5 (NP_084090.1) or N-terminal HA-tagged CHMP5 was amplified by PCR and cloned into pcDNA3.1 (ThermoFisher #V79020) or pHAGE (Harvard) vectors.



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